
Mushroom Extraction Methods
Compare hot water, ethanol, dual, ultrasound-assisted and supercritical extraction and learn how process choices shape extract composition.
An overview of what you will study and build.
This course compares the main ways of turning mushrooms into extracts: hot water, ethanol, dual extraction, ultrasound, microwave and enzyme-assisted methods, and supercritical CO2. You will learn how drying and milling prepare raw material, how solvent, temperature, time and particle size affect yield and composition, and how extracts are concentrated, dried and described with ratios that are often misunderstood.
The word 'extract' covers products that can differ enormously in what they contain. Producers, herbalists and product developers need to understand why a hot water extract rich in polysaccharides behaves differently from an ethanolic extract richer in triterpenes, and how to describe both honestly. The course uses worked process examples and data rather than marketing language, and keeps safety and scale in view at every step. In the final project you will design an extraction process for a chosen species and target compound group, including a flow diagram, key parameters, expected composition, extract ratio calculations and a justification of your method choices.
6 modules, one growing line of knowledge.
Hover or tap a module to follow the path from the first session to the final project. Open a module to see the topics it covers.
- Drying methods and moisture targets
- Milling and particle size
- Raw material testing before extraction
- Solubility of fungal polysaccharides
- Time, temperature and solid-to-liquid ratio
- Yield and composition trade-offs
- Ethanol concentration and triterpene recovery
- Sequential and combined dual extraction
- Solvent safety and residues
- Ultrasound-assisted extraction
- Microwave-assisted extraction
- Enzyme pre-treatment of cell walls
- Principles of supercritical fluids
- Co-solvents and selectivity
- Equipment, costs and applications
- Evaporation and concentration
- Spray drying and carriers
- Understanding and declaring extract ratios
Mushroom Extraction Methods
By the end you will be able to:
- Choose an extraction method suited to a target compound class.
- Explain how process parameters influence extract yield and composition.
- Calculate and correctly interpret native and drug-to-extract ratios.
- Compare the costs and constraints of conventional and advanced techniques.
- Draft a documented extraction process flow for a mushroom ingredient.
Where this programme sits in the school.
Where this programme can take you.
Roles and uses that build on what you learn here. Outcomes depend on your experience and local market.
- Extraction technician in a botanical or mushroom ingredient plant
- Small-batch producer of mushroom tinctures and extracts
- Product developer specifying extracts for supplement brands
- Process assistant in a contract manufacturing facility
- Herbalist producing well-documented in-house preparations
Requirements, assessment and certificate.
Admission requirements
Basic knowledge of medicinal mushrooms and simple chemistry is helpful. Access to kitchen-scale equipment is optional for small practical exercises.
How you are assessed
Progress is assessed through participation in live sessions and module exercises such as ratio calculations and process comparisons. The final process design is reviewed by the professor.
Final project
A documented extraction process design for a chosen species and target compound group.
Certificate
Course Certificate
On completing the programme and its project, Fungaris issues a Course Certificate. Accreditations are assessed according to the training completed and the local regulations of each country.
How the programme grows, phase by phase.
- Raw Material Preparation: Drying and Milling
- Hot Water Extraction of Polysaccharides
- Ethanol and Dual Extraction
- Ultrasound, Microwave and Enzyme-Assisted Extraction
- Supercritical CO2 Extraction
- Concentration, Drying and Extract Ratios
Related searches
Questions about this programme
Do I need experience to study Mushroom Extraction Methods?
Basic knowledge of medicinal mushrooms and simple chemistry is helpful. Access to kitchen-scale equipment is optional for small practical exercises.
How is Mushroom Extraction Methods taught?
Live online, in 6 modules with a working professional, a small group and a final project.
How am I assessed?
Progress is assessed through participation in live sessions and module exercises such as ratio calculations and process comparisons. The final process design is reviewed by the professor.
What is the final project?
A documented extraction process design for a chosen species and target compound group.
What certificate do I get?
On completing the programme and its project, Fungaris issues a Course Certificate. Accreditations are assessed according to the training completed and the local regulations of each country.
What can I study after Mushroom Extraction Methods?
The natural next step is Diploma in Functional & Medicinal Mushrooms.
Read before you start.
Keep branching.
From our species guide.
Join the next intake.
Pay in full with a 20% reduction, or reserve your place with 40% and pay the rest in monthly instalments. All prices are stated in US dollars (USD).

